5 resultados para Identificação fenotípica

em Repositório Institucional da Universidade Estadual de São Paulo - UNESP


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This study used a multi-analytical approach based on traditional microbiological methods for cultivation and isolation of heterotrophic bacteria in the laboratory associated with the molecular identification of the isolates and physicochemical analysis of environmental samples. The model chosen for data integration was supported by knowledge from computational neuroscience, and composed by three modules: (i) microbiological parameters, contemplating taxonomic data obtained from the partial sequencing of the 16S rRNA gene from 80 colonies of heterotrophic bacteria isolated by plating method in PCA media. For bacterial colonies isolation were used water samples from Atibaia and Jaguarí rivers collected at the site of water captation for use in effluent treatment, upstream from the entrance of treated effluent from the Paulínia refinery (REPLAN/Petrobras) located in the Paulínia-SP municipality, from the output of the biological treatment plant with stabilization pond and from the raw refinery wastewater; (ii) chemical parameters, ending measures of dissolved oxygen (DO), chemical oxygen demand (COD), biochemical oxygen demand (BOD), chloride, acidity CaCO3, alkalinity, ammonia, nitrite, nitrate, dissolved ions, sulfides, oils and greases; and (iii) physical parameters, comprising the pH determination, conductivity, temperature, transparency, settleable solids, suspended and soluble solids, volatile material, remaining fixing material (RFM), apparent color and turbidity. The results revealed interesting theoretical relationships involving two families of bacteria (Carnobacteriaceae and Aeromonadaceae). Carnobacteriaceae revealed positive theoretical relationships with COD, BOD, nitrate, chloride, temperature, conductivity and apparent color and negative theoretical relationships with the OD. Positive theoretical relationships were shown between Aeromonadaceae and OD and nitrate, while this bacterial family showed negative theoretical...

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The education designed and planned in a clear and objective manner is of paramount importance for universities to prepare competent professionals for the labor market, and above all can serve the population with an efficient work. Specifically, in relation to engineering, conducting classes in the laboratories it is very important for the application of theory and development of the practical part of the student. The planning and preparation of laboratories, as well as laboratory equipment and activities should be developed in a succinct and clear way, showing to students how to apply in practice what has been learned in theory and often shows them why and where it can be used when they become engineers. This work uses the MATLAB together with the System Identification Toolbox and Arduino for the identification of linear systems in Linear Control Lab. MATLAB is a widely used program in the engineering area for numerical computation, signal processing, graphing, system identification, among other functions. Thus the introduction to MATLAB and consequently the identification of systems using the System Identification Toolbox becomes relevant in the formation of students to thereafter when necessary to identify a system the base and the concept has been seen. For this procedure the open source platform Arduino was used as a data acquisition board being the same also introduced to the student, offering them a range of software and hardware for learning, giving you every day more luggage to their training

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)